CHROMOSOME COUNTS OFCOMPOSITAE FROMECUADOR ANDVENEZUELA'David M. Spooner,"2Diederik C. D. De Jong,AByung-Yun Sun,� Tod F. Stuessy,5Karla M. Gengler�, Guy L. Nesom,"'and Paul E. Berry7AIs'TR ACT Forty-three chromosome counis of Compositae arc reported from Ecuador and Venezuela. A lirst generic count isreported for Stuckertiella Beauverd (n = 11-12). and lirsl counts also are reported for 15 species in Ageratirui Spach,Chromolaena DC., Coespeletia Cuatrec., Erigeron I.. EseI'ltia Mutis. Gynoxys Cass., Hinterhubera Sch. Bip., Oritro-phium (HBK) Cuatrec., Pentaalia Cass., Ruilop<'zia Cuatrec.. Senecio L., 7anacetum L.., and VsLquezia Phil. Additional(counls also are provided for 23 populations of previously counted taxa, two of which are new numbers. The taxonomicimplications of certain of these cournts are discussed. During research expeditions to Ecuador andVenezuela in the past 15 years, we have collectednumerous samples of Compositae. In addition toherbariumn material, we have obtained floral budsfor chromosomal studies. This paper gives the re-sults of these studies, which are important for help-ing delimit taxa and reconstructing phylogeny inthe fanily. These investigations extend previouschroniosomal work by Keil & Stuessy (1975, 1977),Jansen & Stuessy (1980), and Jansen et al. (1984).This paper lists first counts for genera. species, andvarieties, as well as confirinatory data for other taxaknown front only one or a few reports. Discussionfocuses on taxonomic and/or evolutionary implica-tions.MATrERIAIS ANDI) METIODS The materials and methods involving convention-al squash techniques for meioltic stages of nuclei inipollen parent cells are outlined in Keil & Stuessy(1975, 1977) and Jansen & Stuessy (1980). EitherSnow's stain (Snow, 1963) or acetocarmine wasused. Voucher specimnens of Venezuelan collectionsare deposited at Instituto Bot�nico. Venezuela(VEN). and Ecuadorian collections are at OhioState University (OS).RESUI iTS The 42 new chromosome counts are listed in Ta-ble 1. First counts are reported for 1 genus and 17species, and 23 additional counts are for previouslyreported taxa, 2 of which are new numbers. Thenew generic count is for Stuckertiella Beauverd.New species counts are in Ageratina Spach,Chromolaena DC., Coespeletia Cuatree., ErigeronL., Espeletia Mutis, Gynoxys Cass., HinterhuberaSch. Bip., Oritrophium (HBK) Cuatrec., PentacaliaCass.. Ruilopezia Cuatrec.. Senecio L., TanacetumL., and Vasquezia Phil.DISCUSSION The discussion here is restricted to first or newcounts and to significant comments, which is theapproach used in previous papers (Keil & Stuessy,1975. 1977; Jansen & Stuessy, 1980; Jansen et al.,1984). References for statements regarding rangesof chromnosomal variation within genera or for fre-quently counted species will not be given; docu-mentation for those counts cornes from availablechromosomal indices (Darlington & Wylie, 1955;Cave. 1958-65; Oniduff, 1967-69; Fedorov, 1969;Moore, 1970-77; Goldblatt, 1981-1988; Goldblatt& Johnson, 1990-1991). ' This study was supported by NSF grants DEB 7520819 and INT 7684454 to TFS. We thank the Korean Scienceand Engineering Foundation for a postdoctoral fellowship for BYS for a research visit to Ohio State University, and theGraduate School of Ohio State for a postdoctoral fellowship for GLN. 2 USDA, ARS Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, U.S.A. Dl)epartment of Biology, Raymond Walters College, University of Cincinnati, Cincinnati, Ohio 45236, U.S.A. IDepartment of Biology, Chonbuk National University, Chonju 560-756, Korea. l)epartment of Plant Biology, Ohio State University, Columbus, Ohio 43210, U.S.A. Present address: The NaturalHlistory Museum of Los Angeles County, 900 Exposition Boulevard, l)os Angeles, California 9(XX)7, U.S.A. " erbarium, University of Texas, Austin, Texas 78713, U.S.A. Missouri Botanical Garden. St. louis. Missouri 63166. U.S.A.ANN. MIssoURI BOT. (GARD. 82: 596-602. 1995.
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